lyn fak fret biosensor plasmid  (Addgene inc)

Journal: Materials Today Bio

Article Title: Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling

doi: 10.1016/j.mtbio.2026.102908

Figure Lengend Snippet: TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using a Lyn–FAK FRET biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The Lyn–FAK FRET biosensor plasmid (Plasmid #78299, Addgene, Watertown, MA, USA) was inserted into the pAd/CMV/V5-DESTTM GatewayTM vector (V49320, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions to generate an adenoviral expression construct.

Techniques: Activation Assay, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Inhomogeneous Tau polymerization, core–shell organization, and seed formation during Tau condensate aging

doi: 10.64898/2026.03.18.711671

Figure Lengend Snippet: (A) Schematic of seeding Tau aggregation in Tau biosensor (HEK293 expressing TauRD P301S -CFP) cells by aged Tau/RNA condensates. (B) Example images of Tau biosensor cells seeded, or not, with 24 h-old Tau/RNA condensates. Scale bars = 50 μm. (C) High-resolution imaging and 3D-reconstruction of TauRD P301S -CFP in condensate seeded Tau biosensor cells, with counterstaining of the nuclear envelope by Lamin B1 immunostaining shows subcellular positioning of seeded Tau species: Many small Tau foci form in the cytosol and some at the nuclear envelope, larger cytoplasmic Tau aggregates are positioned close to the nucleus, and some Tau clusters also form in the nucleus. Scale bars = 2 μm. (D) Confocal time course imaging of Tau biosensor cells upon seeding with 24 h-old Tau/RNA condensates. Images show sequential formation of Tau accumulation in the same cell: first, cytosolic Tau foci (CLUS) form, followed by Tau foci at the nuclear envelope (NE), larger cytoplasmic Tau aggregates (CYT) close to the nucleus, and, finally, intranuclear circular Tau aggregates (NUC) can be observed. (E) Quantification Tau accumulation types from time course imaging experiments. For analysis, cytoplasmic CLUS and CYT were combined. n=21 analyzed time course series (z-stack), data shown as mean±SEM, one-way ANOVA with Tukey post-test for percentage at 21 h for each accumulation class. (F) STED microcopy of seeded Tau biosensor cells, counter stained with SiR-tubulin (left panel) or immunostained for Lamin B1 (right panel), showing different Tau accumulation types. Zoom-ins show elongated cytosolic Tau structures adjacent to microtubules (left) and Tau foci at the outer nuclear envelope (right). Position of nuclei are indicated by white stars, inner nuclear envelope-nucleoplasm border is indicated by white, dashed lines. Scale bars = 5 μm in overview and 1 μm in zoom-ins. (G) Principle of CFP lifetime FLIM in Tau biosensor cells expressing TauRD P301S -CFP or TauRD P301S -CFP and TauRD P301S -YFP (TauRD P301S -CFP/YFP). CFP lifetime is quenched by molecular crowding in TauRD P301S -CFP accumulations and by both molecular crowding and Tau-Tau interactions in TauRD P301S -CFP/YFP accumulations. (H) Example images of seeded Tau biosensor cells (top: TauRD P301S -CFP cells; bottom: TauRD P301S -CFP/YFP cells). CFP intensity is shown, as well as CFP lifetime components, fit-free defined based on ROIs in phasor plots), superimposed on CFP intensity. Lifetime components could be defined for free soluble Tau (LT SOL , pink), Tau foci in cytosol (CLUS) and at the nuclear envelope (NE; LT CLUS+NE ), cytosolic (CYT) and nuclear (NUC; LT CYT+NUC ) Tau aggregates, and amyloid-like cytosolic Tau aggregates (AMY; LT AMY ). Scale bars = 5 μm. (I) Lifetimes of Tau accumulation types in TauRD P301S -CFP and TauRD P301S -CFP/YFP accumulations. Data shown as mean±SD, comparison of Tau accumulation types within cell type: one-way ANOVA with Tukey post-test. (J) FRET contribution to CFP lifetime quenching in seeded TauRD P301S -CFP/YFP cells, estimated by subtracting lifetimes of Tau accumulation types measured in TauRD P301S -CFP/YFP cells from that measured in TauRD P301S -CFP cells. % values give the proportion of plotted values to entire CFP lifetime quenching in TauRD P301S -CFP/YFP cells. Data shown as mean±SD. (K) Examples of ODT overlaid with correlative fluorescent image of seeded and unseeded TauRD P301S -CFP/YFP cells. (L) Quantification of densities (mg/ml) determined from RI tomograms for Tau accumulations (CYT, NUC) and subcellular compartments (cytoplasm, nucleoplasm, nuclear envelope, and nucleolus). Note, nuclear envelope density in seeded Tau biosensor cells was determined as proxy for Tau foci at the nuclear envelope. n = 15-66 measurements, box plot shows full data range (Min to Max) with all data points, line indicates median, cross indicates mean. Comparison within aggregate type and subcellular compartments: one-way ANOVA with Tukey post-test, or Student T-test for nuclear envelope.

Article Snippet: HEK293 cells stably expressing the Tau repeat domain (TauRD) containing the frontotemporal dementia (FTD)-mutation P301S and fused to CFP or YFP (Tau biosensor cells; TauRD P301S -CFP/YFP); ATCC #CRL-3275; cells provided by Marc Diamond through Erich Wanker) were grown in 8-well imaging dishes (Ibidi).

Techniques: Expressing, Imaging, Immunostaining, Staining, Comparison